Ab Initio Multiple Cloning Method for Non-Adiabatic

An Ab Initio A number of Cloning Technique for Non-Adiabatic Excited-State Molecular Dynamics in NWChem

The lately developed ab initio a number of cloning (AIMC) method based mostly on the multiconfigurational Ehrenfest (MCE) technique offers a strong and correct approach of describing the excited-state dynamics of molecular programs. The AIMC technique is a managed approximation to nonadiabatic dynamics with a specific power within the correct description of decoherence results due to the branching of vibrational wavepackets at a degree crossing. Right here, we report a brand new implementation of the AIMC algorithm within the open supply NWChem computational chemistry program.

The framework combines linear-response time-dependent density purposeful principle with Ehrenfest mean-field principle to find out the equations of movement for classical trajectories. The multidimensional wave operate is decomposed right into a superposition of Gaussian coherent states guided by Ehrenfest trajectories (i.e., MCE method), which may clone with totally quantum mechanical amplitudes and phases. By utilizing an environment friendly time-derivative based mostly nonadiabatic coupling method throughout the AIMC technique, all observables are calculated on-the-fly within the nonadiabatic molecular dynamics course of.

As a consultant instance, we apply our implementation to review the ultrafast photoinduced digital and vibrational vitality switch in a pyridine molecule. The results of the cloning process on digital and vibrational coherence, leisure and unidirectional vitality switch are mentioned. This new AIMC implementation offers a high-level nonadiabatic molecular dynamics framework for simulating photoexcited dynamics in complicated molecular programs and experimentally related ultrafast spectroscopic probes, akin to nonlinear coherent optical and X-ray indicators.

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Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only

MC101A-1 50 reactions
EUR 1056

Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only

MC096A-1 10 reactions
EUR 1856

Fast, Simple & Efficient Cloning Kit without competent cell

GWB-PS10F8 20reactions Ask for price

Fast, Simple & Efficient Cloning Kit without competent cell

GWB-PSEBB3 40reactions Ask for price

Fast, Simple & Efficient Cloning Kit without competent cell

GWB-6F5A1D 100reactions Ask for price

Fast, Simple & Efficient Cloning Kit without competent cell

GWB-768936 10reactions Ask for price

Quick PCR™ Plus Assembly Kit with Competent Cells

78532-1 10 reactions
EUR 195
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).

Quick PCR™ Plus Assembly Kit with Competent Cells

78532-2 50 reactions
EUR 850
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).

Fast and Efficient Mutagenesis Kit without Competent Cells

GWB-PS0724 10reactions Ask for price

Fast and Efficient Mutagenesis Kit without Competent Cells

GWB-PS1E05 20reactions Ask for price

Fast and Efficient Mutagenesis Kit without Competent Cells

GWB-PSB5BB 100reactions Ask for price

Fast and Efficient Mutagenesis Kit without Competent Cells

GWB-PSDBF3 40reactions Ask for price

*E.coli BL21(DE3) Competent Cells

IECBL21CC1ML each
EUR 119
Description: *E.coli BL21(DE3) Competent Cells

*E. coli HB101 Competent Cells

IECHB101CC1ML each
EUR 119
Description: *E. coli HB101 Competent Cells

*E. coli JM109 Competent Cells

IECJM109CC1ML each
EUR 119
Description: *E. coli JM109 Competent Cells

Zenoquick Competent E. coli Transformation Kit

Z6001-001 1Kit
EUR 295.2

*E.coli DH5-alpha Competent Cells

IECDH5ACC1ML each
EUR 119
Description: *E.coli DH5-alpha Competent Cells

Chemically Competent Cell

20-abx098066
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  • 1 ml
  • 2 ml

Chemically Competent Cell

abx098066-100l 100 µl
EUR 618.75

Chemically Competent Cell

abx098066-200l 200 µl
EUR 687.5

Chemically Competent Cell

abx098067-100l 100 µl
EUR 675

Chemically Competent Cell

abx098067-200l 200 µl Ask for price

ProClone™ Competent Cells

E003 5 ml (4 x 1.25ml)
EUR 150
Description: ProClone™ Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm’s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/µg can be achieved using abm’s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone™ Competent Cells the premier choice for both routine and challenging subcloning projects.

Ar.A4 Chemically Competent Cell

ACC-116 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.A4 Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, carrot, licorice and other plants.

DMT Chemically Competent Cell

20-abx098071
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  • 1 ml
  • 500 ul

DB3.1 Chemically Competent Cell

abx098865-1ml 1 ml
EUR 543.6

DMT Chemically Competent Cell

abx098071-100l 100 µl
EUR 618.75

DMT Chemically Competent Cell

abx098071-200l 200 µl
EUR 662.5

DB3.1 Chemically Competent Cell

abx098865-100l 100 µl
EUR 362.5

DB3.1 Chemically Competent Cell

abx098865-200l 200 µl
EUR 425

Single Step Ultra Competent Cell Preps Kit

BS523 25ml
EUR 91.32

Single Step Ultra Competent Cell Preps Kit

BS524 50ml
EUR 112.2

AGL1 Chemically Competent Cell

ACC-105 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: AGL1 Chemically Competent Cell is suitable for transgenic operations of rice, Arabidopsis, poplar and other plants.

Ar.Qual Chemically Competent Cell

ACC-117 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.Qual Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tomato, citrus and other plants.

K599 Chemically Competent Cell

ACC-121 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: K599 Chemically Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.

Chemically Competent Cell (StrR)

20-abx098064
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  • 1 ml
  • 2 ml

BL21 Chemically Competent Cell

20-abx098105
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  • 1 ml
  • 2 ml
  • 500 ul

Chemically Competent Cell (StrR)

abx098064-100l 100 µl
EUR 618.75

Chemically Competent Cell (StrR)

abx098064-200l 200 µl
EUR 675

BL21 Chemically Competent Cell

abx098105-100l 100 µl
EUR 662.5

BL21 Chemically Competent Cell

abx098105-200l 200 µl
EUR 712.5

C58C1 Chemically Competent Cell

ACC-119 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: C58C1 Chemically Competent Cell is suitable for transgenic operations of rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco and other plants.

TOP10 Chemically Competent Cell

ACC-129 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price

Competent Cells, Gold Efficiency

BIO-85027 1ml ( 20 x 50µl ) Ask for price

Stbl3 Chemically Competent Cell

abx098072-100l 100 µl
EUR 675

Stbl3 Chemically Competent Cell

abx098072-1ml 1 ml Ask for price

Stbl3 Chemically Competent Cell

abx098072-200l 200 µl
EUR 737.5

GV3101 Chemically Competent Cell

ACC-100 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: GV3101 Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

EHA105 Chemically Competent Cell

ACC-103 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA105 Chemically Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.

EHA101 Chemically Competent Cell

ACC-108 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA101 Chemically Competent Cell is suitable for transgenic operations of corn, rice, tobacco and other plants.

MSU440 Chemically Competent Cell

ACC-118 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: MSU440 Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tea tree, artemisia annua and other plants.

HiPurA® DH5α Competent Cells

MBT111-10R 1 unit
EUR 24.46
Description: HiPurA® DH5α Competent Cells

HiPurA® DH5α Competent Cells

MBT111-20R 1 unit
EUR 38.47
Description: HiPurA® DH5α Competent Cells

HiPurA® Dh5α Competent Cells

MBT111-40R 1 unit
EUR 69.82
Description: HiPurA® Dh5α Competent Cells

BirA-transformed Competent E. coli cells

27462 10 vials
EUR 490
Description: BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials._x000D_Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA_x000D_expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene._x000D_These cells are compatible with most cloning vectors and are suitable for expression and_x000D_biotinylation of recombinant proteins using AviTag™ technology.

LBA4404 Chemically Competent Cell

ACC-107 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: LBA4404 Chemically Competent Cell is suitable for transgenic operations of tomatoes, tobacco and other plants.

Ar.A4 Electroporation Competent Cell

ACC-122 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: Ar.A4 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, carrot, licorice and other plants.

Competent Cells, Bronze Efficiency

BIO-85025 2ml ( 10 x 200µl) Ask for price

Competent Cells, Silver Efficiency

BIO-85026 2ml ( 10 x 200µl ) Ask for price

Ar.1193 Chemically Competent Cell

ACC-120 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.1193 Chemically Competent Cell is suitable for transgenic operations of leguminosae, solanaceae and other plants.

AGL1 Electroporation Competent Cell

ACC-113 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: AGL1 Electroporation Competent Cell is suitable for transgenic operations of rice, Arabidopsis, poplar and other plants.

Ar.Qual Electroporation Competent Cell

ACC-123 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: Ar.Qual Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, tomato, citrus and other plants.

K599 Electroporation Competent Cell

ACC-127 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: K599 Electroporation Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.

HT115 Agrobacterium Competent Cells

ACC-130 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price

C58C1 Electroporation Competent Cell

ACC-125 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: C58C1 Electroporation Competent Cell is suitable for transgenic operations of rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco and other plants.

Endogenous retrovirus (replication competent) PCR kit

PCR-H725-48D 48T
EUR 543.6

Endogenous retrovirus (replication competent) PCR kit

PCR-H725-96D 96T
EUR 686.4

Endogenous retrovirus (replication competent) PCR kit

PCR-V725-48D 50T
EUR 510.96
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit

PCR-V725-96D 100T
EUR 625.8
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

5 alpha Chemically Competent Cell

20-abx098065
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  • 1 ml
  • 2 ml

BL21 (DE3) Chemically Competent Cell

20-abx098101
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  • 1 ml
  • 2 ml
  • 500 ul

DE3 Chemically Competent Cell (CamR)

20-abx098103
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  • 1 ml
  • 2 ml
  • 500 ul

5 alpha Chemically Competent Cell

abx098065-100l 100 µl
EUR 618.75

5 alpha Chemically Competent Cell

abx098065-200l 200 µl
EUR 675

BL21 (DE3) Chemically Competent Cell

abx098101-100l 100 µl
EUR 662.5

BL21 (DE3) Chemically Competent Cell

abx098101-200l 200 µl
EUR 712.5

DE3 Chemically Competent Cell (CamR)

abx098103-100l 100 µl
EUR 675

DE3 Chemically Competent Cell (CamR)

abx098103-200l 200 µl
EUR 725

GV3101 Electroporation Competent Cell

ACC-109 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: GV3101 Electroporation Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

EHA105 Electroporation Competent Cell

ACC-112 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: EHA105 Electroporation Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.

EHA101 Electroporation Competent Cell

ACC-115 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: EHA101 Electroporation Competent Cell is suitable for transgenic operations of corn, rice, tobacco and other plants.

MSU440 Electroporation Competent Cell

ACC-124 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: MSU440 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, tea tree, artemisia annua and other plants.

Blue Chemically Competent Cell (TetR)

20-abx098068
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  • 1 ml
  • 2 ml

Blue Chemically Competent Cell (TetR)

abx098068-100l 100 µl
EUR 618.75

Blue Chemically Competent Cell (TetR)

abx098068-200l 200 µl
EUR 687.5

LBA4404 Electroporation Competent Cell

ACC-114 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: LBA4404 Electroporation Competent Cell is suitable for transgenic operations of tomatoes, tobacco and other plants.

HiPurA® BL21 (DE3) Competent Cells

MBT143-10R 1 unit
EUR 24.46
Description: HiPurA® BL21 (DE3) Competent Cells

HiPurA® BL21 (DE3) Competent Cells

MBT143-20R 1 unit
EUR 38.47
Description: HiPurA® BL21 (DE3) Competent Cells

HiPurA® BL21 (DE3) Competent Cells

MBT143-40R 1 unit
EUR 69.82
Description: HiPurA® BL21 (DE3) Competent Cells

AGL1 (pSoup) Chemically Competent Cell

ACC-106 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: AGL1 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

Ar.1193 Electroporation Competent Cell

ACC-126 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: Ar.1193 Electroporation Competent Cell is suitable for transgenic operations of leguminosae, solanaceae and other plants.

ATCC15834 Electroporation Competent Cell

ACC-128 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: ATCC15834 Electroporation Competent Cell is suitable for transgenic operations of gramineae, leguminous, tobacco and other plants.

HiPurA® XL1-Blue Competent Cells

MBT144-10R 1 unit
EUR 24.46
Description: HiPurA® XL1-Blue Competent Cells

HiPurA® XL1-Blue Competent Cells

MBT144-20R 1 unit
EUR 38.47
Description: HiPurA® XL1-Blue Competent Cells

HiPurA® XL1-Blue Competent Cells

MBT144-40R 1 unit
EUR 69.82
Description: HiPurA® XL1-Blue Competent Cells

GV3101 (pSoup) Chemically Competent Cell

ACC-101 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: GV3101 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

EHA105 (pSoup) Chemically Competent Cell

ACC-104 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA105 (pSoup) Chemically Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.

BL21 Chemcially Competent Cells - 24x50µl

1041-24 24/PK
EUR 190
Description: Intact Genomics BL21 chemically competent cells are suitable for transformation and routine protein expression from non-T7 vectors.Product Includes:BL21 competent cellspUC19 control DNARecovery medium

OORA00274-100ML - Competent Cell Buffer

OORA00274-100ML 100mL
EUR 159

Endogenous retrovirus (replication competent) RT PCR kit

RTq-H725-100D 100T
EUR 860.4

Endogenous retrovirus (replication competent) RT PCR kit

RTq-H725-150D 150T
EUR 969.6

Endogenous retrovirus (replication competent) RT PCR kit

RTq-H725-50R 50T
EUR 1155.6

Endogenous retrovirus (replication competent) RT PCR kit

RTq-V725-100D 100T
EUR 754.2
Description: A Real-Time PCR kit for detection of Endogenous retrovirus (replication competent) .

Endogenous retrovirus (replication competent) RT PCR kit

RTq-V725-150D 150T
EUR 841.2
Description: A Real-Time PCR kit for detection of Endogenous retrovirus (replication competent) .

Endogenous retrovirus (replication competent) RT PCR kit

RTq-V725-50R 50T
EUR 757.68
Description: A Real-Time PCR kit for detection of Endogenous retrovirus (replication competent) .

DH10B Chemically Competent Cells - 12x50µl

1011-12 12/PK
EUR 125
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium

DH10B Chemically Competent Cells - 24x50µl

1011-24 24/PK
EUR 205
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium

DH10B Chemically Competent Cells - 6x100µl

1012-12 6/PK
EUR 115
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium

DH10B Chemically Competent Cells - 6x200µl

1014-24 6/PK
EUR 165
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium

HB101 Chemically Competent Cells - 6x100µl

1071-12 100μL
EUR 99
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening

JM109 Chemically Competent Cells - 6x200µl

1062-24 200μL
EUR 149
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.

DH10B Chemically Competent Cells - 12x100µl

1012-24 12/PK
EUR 195
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium

DH10B Chemically Competent Cells - 24x100µl

1012-48 24/PK
EUR 330
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium

DH10B Chemically Competent Cells - 12x200µl

1014-48 12/PK
EUR 310
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium

HB101 Chemically Competent Cells - 24x100µl

1071-24 100μL
EUR 315
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening

MG1655 Chemically Competent Cells - 12x50µl

1076-12 50μL
EUR 190
Description: Intact Genomics (ig®) chemically competent MG1655 chemically competent cells are suitable for routine DNA transformation and other research purposes. MG1655 is the “wild type” K-12 strain with minimal genetic manipulation having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange

JM109 Chemically Competent Cells - 12x200µl

1062-48 200μL
EUR 255
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.

DE3 Chemically Competent Cell (KanR, TetR)

abx098104-100l 100 µl
EUR 675

DE3 Chemically Competent Cell (KanR, TetR)

abx098104-200l 200 µl Ask for price

Blue Chemically Competent Cell (TetR, CamR)

20-abx098069
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  • Ask for price
  • 1 ml
  • 2 ml

BL21 (DE3) pLysS Chemically Competent Cell

20-abx098102
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  • Ask for price
  • Ask for price
  • 1 ml
  • 2 ml
  • 500 ul

GV3101 (pSoup) Electroporation Competent Cell

ACC-110 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: GV3101 (pSoup) Electroporation Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

Frozen Storage Buffer for Competent Cells

40620000-1 125 mL
EUR 60.54

Frozen Storage Buffer for Competent Cells

40620000-2 250 mL
EUR 115.12

Chemically Competent Agrobacterium Combo Pack

1091-12 50μL
EUR 945
Description: Intact Genomics Chemically Competent Agrobacterium Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research. The ElectroCompetent Agrobacterium Combo Pack contains 3 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia and our proprietary Agro Recovery Media.The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.

DL39 (DE3) Chemically Competent Cells - 12x50µl

1061-12 12/PK
EUR 382.8

DL39 (DE3) Chemically Competent Cells - 24x50µl

1061-24 24/PK
EUR 621.6

DL39 (DE3) Chemically Competent Cells - 6x200µl

1064-24 200μL
EUR 465
Description: Intact Genomics chemically competent DL39 (DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues. DL39 (DE3) can also be used to reduce NMR cross-labeling via transaminase activity for valine, leucine, isoleucine, aspartate, phenylalanine, tyrosine and tryptophan residuesProduct Includes:DL39 (DE3) competent cellspUC19 control DNARecovery medium

BL21(DE3) Chemically Competent Cells - 12x50µl

1051-12 12/PK
EUR 120
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium

BL21(DE3) Chemically Competent Cells - 24x50µl

1051-24 24/PK
EUR 200
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium

BL21(DE3) Chemically Competent Cells - 6x200µl

1054-24 6/PK
EUR 145
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium

AGL1 Chemically Competent Agrobacterium - 6x50µl

1083-06 50μL
EUR 355
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

JM109(DE3) Chemically Competent Cells - 6x200µl

1063-24 200μL
EUR 149
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.

BL21(DE3) Chemically Competent Cells - 12x200µl

1054-48 12/PK
EUR 270
Description: Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.Product Includes:BL21 (DE3) competent cellspUC19 control DNARecovery medium

GV3101 (pSoup-p19) Chemically Competent Cell

ACC-102 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: GV3101 (pSoup-p19) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

T1 Phage Resistant Chemically Competent Cell

abx098070-100l 100 µl
EUR 650

T1 Phage Resistant Chemically Competent Cell

abx098070-200l 200 µl
EUR 700

Chemically Competent Cell (106 cfu / mu g DNA)

20-abx098067
  • Ask for price
  • Ask for price
  • 1 ml
  • 500 ul

AGL1 Chemically Competent Agrobacterium - 18x50µl

1083-18 50μL
EUR 755
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

C58C1 Chemically Competent Agrobacterium - 6x50µl

1086-06 50μL
EUR 355
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium

JM109(DE3) Chemically Competent Cells - 12x200µl

1063-48 200μL
EUR 255
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.

MSU440 Chemically Competent Agrobacterium- 6x50µl

1077-12 50μL
EUR 355
Description: Intact Genomics (ig®) MSU440 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(str R) MSU440 Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. MSU440 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants. MSU440 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin resistance.

GV3101 Chemically Competent Agrobacterium - 6x50µl

1082-06 50μL
EUR 355
Description: GV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.Product Includes:• GV3101 Chemically cells• pCAMBIA control DNA• Recovery medium

EHA105 Chemically Competent Agrobacterium - 6x50µl

1084-06 50μL
EUR 355
Description: Intact Genomics EHA105 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.Product Includes:• EHA105 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

C58C1 Chemically Competent Agrobacterium - 18x50µl

1086-18 50μL
EUR 755
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium

DH5-Alpha Chemically Competent Cells - 12x50µl

1031-12 12/PK
EUR 130
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of  the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium

DH5-Alpha Chemically Competent Cells - 24x50µl

1031-24 24/PK
EUR 195
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of  the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium

DH5-Alpha Chemically Competent Cells - 6x100µl

1032-12 6/PK
EUR 120
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of  the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium

DH5-Alpha Chemically Competent Cells - 6x200µl

1034-24 6/PK
EUR 150
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of  the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium

Endogenous retrovirus (replication competent) One-Step PCR kit

Oneq-V725-100D 100T
EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Endogenous retrovirus (replication competent).

Endogenous retrovirus (replication competent) One-Step PCR kit

Oneq-V725-150D 150T
EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Endogenous retrovirus (replication competent).

Endogenous retrovirus (replication competent) One-Step PCR kit

Oneq-V725-50D 50T
EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Endogenous retrovirus (replication competent).

Endogenous retrovirus (replication competent) One-Step PCR kit

Oneq-H725-100D 100T
EUR 1039.2

Endogenous retrovirus (replication competent) One-Step PCR kit

Oneq-H725-150D 150T
EUR 1177.2

Endogenous retrovirus (replication competent) One-Step PCR kit

Oneq-H725-50D 50T
EUR 861.6

MSU440 Chemically Competent Agrobacterium- 18x50µl

1077-36 50μL
EUR 755
Description: Intact Genomics (ig®) MSU440 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(str R) MSU440 Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. MSU440 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants. MSU440 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin resistance.

GV3101 Chemically Competent Agrobacterium - 18x50µl

1082-18 50μL
EUR 755
Description: GV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.Product Includes:• GV3101 Chemically cells• pCAMBIA control DNA• Recovery medium

EHA105 Chemically Competent Agrobacterium - 18x50µl

1084-18 50μL
EUR 755
Description: Intact Genomics EHA105 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.Product Includes:• EHA105 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

LBA4404 Chemically Competent Agrobacterium - 6x50µl

1085-06 50μL
EUR 355
Description: Intact Genomics LBA4404 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.Product Includes:• LBA4404 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

DH5-Alpha Chemically Competent Cells - 12x100µl

1032-24 12/PK
EUR 200
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of  the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium

DH5-Alpha Chemically Competent Cells - 24x100µl

1032-48 24/PK
EUR 335
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of  the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium

Ar. A4 Chemically Competent Agrobacterium- 6x50µl

1072-06 50μL
EUR 355
Description: Intact Genomics (ig®) Ar.A4 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(kan R) Ar A4 Ri(agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. Ar.A4 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, carrot and other plants. Ar.A4 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays kanamycin resistance.

DH5-Alpha Chemically Competent Cells - 12x200µl

1034-48 12/PK
EUR 315
Description: Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig™ 5-alpha chemically competent cells are at least twice the transformation efficiency of  the nearest competitor.Product Includes:ig™ 5-alpha competent cellspUC19 control DNARecovery medium

LBA4404 Chemically Competent Agrobacterium - 18x50µl

1085-18 50μL
EUR 755
Description: Intact Genomics LBA4404 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.Product Includes:• LBA4404 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

Ar. A4 Chemically Competent Agrobacterium- 18x50µl

1072-18 50μL
EUR 755
Description: Intact Genomics (ig®) Ar.A4 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(kan R) Ar A4 Ri(agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. Ar.A4 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, carrot and other plants. Ar.A4 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays kanamycin resistance.

Cloning of a HcCreb gene and evaluation of its results on nacre coloration and melanin synthesis in Hyriopsis cumingii 

Creb (Cyclic AMP response ingredient binding protein) is a nuclear regulatory issue that regulates transcription by means of autophosphorylation. In melanocytes, cAMP’s corresponding components bind to the Creb protein to autophosphorylation and activate MITF (Microphthalmia-associated transcription issue). MITF stimulates Tyrosine(tyr) to induce melanocytes to distinguish into eumelanin and pheomelanin. On this research, a HcCreb gene in Hyriopsis cumingii was cloned and its results on melanin synthesis and nacre coloration had been studied. HcCreb was expressed in each purple and white mussels, and there was a major distinction in expression between adductor muscle (p<0.01) and mantle tissue (p<0.05).

Different tissues didn’t present vital variations (apart from gill tissue), and typically, the extent of mRNA expression was increased in purple mussels than in white mussels. In each white and purple mussels expression ranges in gill tissue was the best, adopted by the mantle. Robust and particular mRNA indicators had been detected within the dorsal epithelial cells of the mantle pallial layer, indicating that HcCreb could also be concerned in nacre formation. After arbutin remedy, the expression of HcCreb decreased considerably. By additional testing the modifications in mantle melanin content material it was discovered that the melanin content material after arbutin remedy decreased considerably in comparison with the management group (p<0.05). It’s speculated that the HcCreb gene performs a task within the strategy of melanin synthesis and nacre coloration formation in H. cumingii.

Glucosides in Biodiesel 

Vegetable oil-derived biodiesels have a significant high quality downside because of the presence of precipitates shaped by steryl glucosides, which clog filters and injectors of diesel engines. An environment friendly, scalable, and cost-effective technique to hydrolyze steryl glucosides utilizing thermostable enzymes has been developed. Right here, strategies to find, categorical in recombinant microorganisms and manufacture enzymes with SGase exercise, in addition to strategies to deal with biodiesel with such enzymes, and to measure the content material of steryl glucosides in biodiesel samples are offered.

Cloning, characterization and expression of a gene encoding endo-1, 4- β-xylanase from the fungus Termitomyces clypeatus 

Enzymatic degradation of hemi-cellulosic substrates has gained loads of industrial attentions lately. Full enzymatic degradation of complicated and recalcitrant hemicellulose requires an enzymatic cocktail consisting primarily of endo-1,4-β-xylanase (xyl), β-xylosidase, arabinofuranosidase and so on. This text studies, for the primary time, the identification, cloning, expression and partial characterization of a potent endo-1,4- β-xylanase gene (pxyl) from the mushroom Termitomyces clypeatus (TC) in E. coli and S. cerevisiae.

The cDNA for pxyl was discovered to be 678 bp that in flip offers rise to a precursor protein (Pxyl) of 225 amino acids lengthy when cloned in prokaryotic expression vector. To characterize moreover, the cDNA was additionally expressed in S. cerevisiae. Bioinformatics research predicted that the Pxyl accommodates a 19 amino acid lengthy chief peptide that permits put up translational modifications together with glycosylation in addition to its environment friendly secretion within the medium. The recombinant protein has been discovered to be a member of GH11 household containing two distant glutamic acids as catalytic residues. This report describes yet one more new and potent supply of xylanase for business exploitation by trade in future.

Cloning, sequence evaluation, and tissue expression of marmoset paraoxonase 1 

Paraoxonase (PON) performs roles within the metabolism of organophosphate xenobiotics and medicines. Regardless of the significance of marmosets for analysis into drug metabolism and pharmacokinetics, marmoset paraoxonase has not but been totally characterised. Consequently, we recognized the PON1 gene within the marmoset genome by sequence homology evaluation. Marmoset PON1 cDNA containing an open studying body (1065 bp) was efficiently cloned from marmoset liver by reverse transcription-polymerase chain response. The deduced amino acid sequence (355 amino acids) has roughly 93% identification with the human ortholog and accommodates vital amino acid residues for substrate binding and calcium ion coordination.

In response to a phylogenetic tree of PON1 amino acid sequences constructed utilizing knowledge from seven animal species, marmoset PON1 is nearer to human PON1 than it’s to the PON1 orthologs of experimental animals akin to pigs, rabbits, rats, and mice. Marmoset PON1 mRNA was predominantly expressed in liver among the many 5 tissues examined. Marmoset PON1 protein secreted into plasma was detected by immunoblotting. The paraoxon-hydrolyzing exercise in plasma was increased in marmosets than in people. Primarily based on these knowledge, we concluded that marmoset and human PON1 have related traits with regard to genomic construction, amino acid sequences, and tissue distribution.

A novel pH and thermo-tolerant halophilic alpha-amylase from average halophile Nesterenkonia sp. pressure F: gene evaluation, molecular cloning, heterologous expression and biochemical characterization 

A novel pH and thermo-tolerate halophilic alpha-amylase from reasonably halophilic bacterium, Nesterenkonia sp.pressure F was cloned and expressed in Escherichia coli. 16S rRNA sequence of the pressure shared 99.46% similarities with carefully associated kind species. Additionally, the genome sequence shared ANI values beneath 92% and dDDH values beneath 52% with the carefully associated kind species. Consequently, it’s proposed that pressure F represents a novel species. The AmyF gene was 1390 bp lengthy and encodes an alpha-amylase of 463 amino acid residues with pI of 4.62. The deduced AmyF shared very low sequence similarity (< 24%) with functionally characterised recombinant halophilic alpha-amylases.

The recombinant alpha-amylase was efficiently purified from Ni-NTA columns with a molecular mass of about 52 KDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme was lively over a variety of temperature (25-75 °C) and pH (4-9) with optimum exercise at 45 °C and seven.5, respectively. Additionally, though it was lively over a numerous concentrations of NaCl and KCl (0-Four M), rising exercise of the enzyme was noticed with rising focus of those salts. Low concentrations of Ca2+ ion had no activating impact, however excessive concentrations of the ion (40-200 mM) enhanced exercise of AmyF.

The enzyme exercise was elevated by rising concentrations of Mg2+, Zn2+, Hg2+ and Fe3+. Nevertheless, it was inhibited solely at very excessive concentrations of those metallic ions. Cu2+ didn’t lower the amylase exercise and the best exercise was noticed at 100 mM of the ion. These properties point out extensive potential purposes of this recombinant enzyme in starch processing industries. That is the primary isolation, cloning and characterization of a gene encoding alpha-amylase from Nesternkonia genus.

Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only

MC101A-1 50 reactions
EUR 1056

Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only

MC096A-1 10 reactions
EUR 1856

Fast, Simple & Efficient Cloning Kit without competent cell

GWB-PS10F8 20reactions Ask for price

Fast, Simple & Efficient Cloning Kit without competent cell

GWB-PSEBB3 40reactions Ask for price

Fast, Simple & Efficient Cloning Kit without competent cell

GWB-6F5A1D 100reactions Ask for price

Fast, Simple & Efficient Cloning Kit without competent cell

GWB-768936 10reactions Ask for price

Quick PCR™ Plus Assembly Kit with Competent Cells

78532-1 10 reactions
EUR 195
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).

Quick PCR™ Plus Assembly Kit with Competent Cells

78532-2 50 reactions
EUR 850
Description: The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).

Fast and Efficient Mutagenesis Kit without Competent Cells

GWB-PS0724 10reactions Ask for price

Fast and Efficient Mutagenesis Kit without Competent Cells

GWB-PS1E05 20reactions Ask for price

Fast and Efficient Mutagenesis Kit without Competent Cells

GWB-PSB5BB 100reactions Ask for price

Fast and Efficient Mutagenesis Kit without Competent Cells

GWB-PSDBF3 40reactions Ask for price

*E.coli BL21(DE3) Competent Cells

IECBL21CC1ML each
EUR 119
Description: *E.coli BL21(DE3) Competent Cells

*E. coli HB101 Competent Cells

IECHB101CC1ML each
EUR 119
Description: *E. coli HB101 Competent Cells

*E. coli JM109 Competent Cells

IECJM109CC1ML each
EUR 119
Description: *E. coli JM109 Competent Cells

Zenoquick Competent E. coli Transformation Kit

Z6001-001 1Kit
EUR 295.2

*E.coli DH5-alpha Competent Cells

IECDH5ACC1ML each
EUR 119
Description: *E.coli DH5-alpha Competent Cells

Chemically Competent Cell

20-abx098066
  • Ask for price
  • Ask for price
  • 1 ml
  • 2 ml

Chemically Competent Cell

abx098066-100l 100 µl
EUR 618.75

Chemically Competent Cell

abx098066-200l 200 µl
EUR 687.5

Chemically Competent Cell

abx098067-100l 100 µl
EUR 675

Chemically Competent Cell

abx098067-200l 200 µl Ask for price

ProClone™ Competent Cells

E003 5 ml (4 x 1.25ml)
EUR 150
Description: ProClone™ Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm’s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/µg can be achieved using abm’s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone™ Competent Cells the premier choice for both routine and challenging subcloning projects.

Ar.A4 Chemically Competent Cell

ACC-116 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.A4 Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, carrot, licorice and other plants.

DMT Chemically Competent Cell

20-abx098071
  • Ask for price
  • Ask for price
  • 1 ml
  • 500 ul

DB3.1 Chemically Competent Cell

abx098865-1ml 1 ml
EUR 543.6

DMT Chemically Competent Cell

abx098071-100l 100 µl
EUR 618.75

DMT Chemically Competent Cell

abx098071-200l 200 µl
EUR 662.5

DB3.1 Chemically Competent Cell

abx098865-100l 100 µl
EUR 362.5

DB3.1 Chemically Competent Cell

abx098865-200l 200 µl
EUR 425

Single Step Ultra Competent Cell Preps Kit

BS523 25ml
EUR 91.32

Single Step Ultra Competent Cell Preps Kit

BS524 50ml
EUR 112.2

AGL1 Chemically Competent Cell

ACC-105 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: AGL1 Chemically Competent Cell is suitable for transgenic operations of rice, Arabidopsis, poplar and other plants.

Ar.Qual Chemically Competent Cell

ACC-117 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.Qual Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tomato, citrus and other plants.

K599 Chemically Competent Cell

ACC-121 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: K599 Chemically Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.

Chemically Competent Cell (StrR)

20-abx098064
  • Ask for price
  • Ask for price
  • 1 ml
  • 2 ml

BL21 Chemically Competent Cell

20-abx098105
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 ml
  • 2 ml
  • 500 ul

Chemically Competent Cell (StrR)

abx098064-100l 100 µl
EUR 618.75

Chemically Competent Cell (StrR)

abx098064-200l 200 µl
EUR 675

BL21 Chemically Competent Cell

abx098105-100l 100 µl
EUR 662.5

BL21 Chemically Competent Cell

abx098105-200l 200 µl
EUR 712.5

C58C1 Chemically Competent Cell

ACC-119 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: C58C1 Chemically Competent Cell is suitable for transgenic operations of rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco and other plants.

TOP10 Chemically Competent Cell

ACC-129 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price

Competent Cells, Gold Efficiency

BIO-85027 1ml ( 20 x 50µl ) Ask for price

Stbl3 Chemically Competent Cell

abx098072-100l 100 µl
EUR 675

Stbl3 Chemically Competent Cell

abx098072-1ml 1 ml Ask for price

Stbl3 Chemically Competent Cell

abx098072-200l 200 µl
EUR 737.5

GV3101 Chemically Competent Cell

ACC-100 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: GV3101 Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

EHA105 Chemically Competent Cell

ACC-103 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA105 Chemically Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.

EHA101 Chemically Competent Cell

ACC-108 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA101 Chemically Competent Cell is suitable for transgenic operations of corn, rice, tobacco and other plants.

MSU440 Chemically Competent Cell

ACC-118 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: MSU440 Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tea tree, artemisia annua and other plants.

HiPurA® DH5α Competent Cells

MBT111-10R 1 unit
EUR 24.46
Description: HiPurA® DH5α Competent Cells

HiPurA® DH5α Competent Cells

MBT111-20R 1 unit
EUR 38.47
Description: HiPurA® DH5α Competent Cells

HiPurA® Dh5α Competent Cells

MBT111-40R 1 unit
EUR 69.82
Description: HiPurA® Dh5α Competent Cells

BirA-transformed Competent E. coli cells

27462 10 vials
EUR 490
Description: BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials._x000D_Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA_x000D_expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene._x000D_These cells are compatible with most cloning vectors and are suitable for expression and_x000D_biotinylation of recombinant proteins using AviTag™ technology.

LBA4404 Chemically Competent Cell

ACC-107 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: LBA4404 Chemically Competent Cell is suitable for transgenic operations of tomatoes, tobacco and other plants.

Ar.A4 Electroporation Competent Cell

ACC-122 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: Ar.A4 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, carrot, licorice and other plants.

Competent Cells, Bronze Efficiency

BIO-85025 2ml ( 10 x 200µl) Ask for price

Competent Cells, Silver Efficiency

BIO-85026 2ml ( 10 x 200µl ) Ask for price

Ar.1193 Chemically Competent Cell

ACC-120 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.1193 Chemically Competent Cell is suitable for transgenic operations of leguminosae, solanaceae and other plants.

AGL1 Electroporation Competent Cell

ACC-113 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: AGL1 Electroporation Competent Cell is suitable for transgenic operations of rice, Arabidopsis, poplar and other plants.

Ar.Qual Electroporation Competent Cell

ACC-123 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: Ar.Qual Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, tomato, citrus and other plants.

K599 Electroporation Competent Cell

ACC-127 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: K599 Electroporation Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.

HT115 Agrobacterium Competent Cells

ACC-130 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price

C58C1 Electroporation Competent Cell

ACC-125 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: C58C1 Electroporation Competent Cell is suitable for transgenic operations of rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco and other plants.

Endogenous retrovirus (replication competent) PCR kit

PCR-H725-48D 48T
EUR 543.6

Endogenous retrovirus (replication competent) PCR kit

PCR-H725-96D 96T
EUR 686.4

Endogenous retrovirus (replication competent) PCR kit

PCR-V725-48D 50T
EUR 510.96
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit

PCR-V725-96D 100T
EUR 625.8
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

5 alpha Chemically Competent Cell

20-abx098065
  • Ask for price
  • Ask for price
  • 1 ml
  • 2 ml

BL21 (DE3) Chemically Competent Cell

20-abx098101
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 ml
  • 2 ml
  • 500 ul

DE3 Chemically Competent Cell (CamR)

20-abx098103
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 ml
  • 2 ml
  • 500 ul

5 alpha Chemically Competent Cell

abx098065-100l 100 µl
EUR 618.75

5 alpha Chemically Competent Cell

abx098065-200l 200 µl
EUR 675

BL21 (DE3) Chemically Competent Cell

abx098101-100l 100 µl
EUR 662.5

BL21 (DE3) Chemically Competent Cell

abx098101-200l 200 µl
EUR 712.5

DE3 Chemically Competent Cell (CamR)

abx098103-100l 100 µl
EUR 675

DE3 Chemically Competent Cell (CamR)

abx098103-200l 200 µl
EUR 725

GV3101 Electroporation Competent Cell

ACC-109 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: GV3101 Electroporation Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

EHA105 Electroporation Competent Cell

ACC-112 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: EHA105 Electroporation Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.

EHA101 Electroporation Competent Cell

ACC-115 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: EHA101 Electroporation Competent Cell is suitable for transgenic operations of corn, rice, tobacco and other plants.

MSU440 Electroporation Competent Cell

ACC-124 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: MSU440 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, tea tree, artemisia annua and other plants.

Blue Chemically Competent Cell (TetR)

20-abx098068
  • Ask for price
  • Ask for price
  • 1 ml
  • 2 ml

Blue Chemically Competent Cell (TetR)

abx098068-100l 100 µl
EUR 618.75

Blue Chemically Competent Cell (TetR)

abx098068-200l 200 µl
EUR 687.5

LBA4404 Electroporation Competent Cell

ACC-114 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: LBA4404 Electroporation Competent Cell is suitable for transgenic operations of tomatoes, tobacco and other plants.

HiPurA® BL21 (DE3) Competent Cells

MBT143-10R 1 unit
EUR 24.46
Description: HiPurA® BL21 (DE3) Competent Cells

HiPurA® BL21 (DE3) Competent Cells

MBT143-20R 1 unit
EUR 38.47
Description: HiPurA® BL21 (DE3) Competent Cells

HiPurA® BL21 (DE3) Competent Cells

MBT143-40R 1 unit
EUR 69.82
Description: HiPurA® BL21 (DE3) Competent Cells

AGL1 (pSoup) Chemically Competent Cell

ACC-106 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: AGL1 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

Ar.1193 Electroporation Competent Cell

ACC-126 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: Ar.1193 Electroporation Competent Cell is suitable for transgenic operations of leguminosae, solanaceae and other plants.

ATCC15834 Electroporation Competent Cell

ACC-128 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: ATCC15834 Electroporation Competent Cell is suitable for transgenic operations of gramineae, leguminous, tobacco and other plants.

HiPurA® XL1-Blue Competent Cells

MBT144-10R 1 unit
EUR 24.46
Description: HiPurA® XL1-Blue Competent Cells

HiPurA® XL1-Blue Competent Cells

MBT144-20R 1 unit
EUR 38.47
Description: HiPurA® XL1-Blue Competent Cells

HiPurA® XL1-Blue Competent Cells

MBT144-40R 1 unit
EUR 69.82
Description: HiPurA® XL1-Blue Competent Cells

GV3101 (pSoup) Chemically Competent Cell

ACC-101 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: GV3101 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

EHA105 (pSoup) Chemically Competent Cell

ACC-104 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA105 (pSoup) Chemically Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.

BL21 Chemcially Competent Cells - 24x50µl

1041-24 24/PK
EUR 190
Description: Intact Genomics BL21 chemically competent cells are suitable for transformation and routine protein expression from non-T7 vectors.Product Includes:BL21 competent cellspUC19 control DNARecovery medium

OORA00274-100ML - Competent Cell Buffer

OORA00274-100ML 100mL
EUR 159

Endogenous retrovirus (replication competent) RT PCR kit

RTq-H725-100D 100T
EUR 860.4

Endogenous retrovirus (replication competent) RT PCR kit

RTq-H725-150D 150T
EUR 969.6

Endogenous retrovirus (replication competent) RT PCR kit

RTq-H725-50R 50T
EUR 1155.6

Endogenous retrovirus (replication competent) RT PCR kit

RTq-V725-100D 100T
EUR 754.2
Description: A Real-Time PCR kit for detection of Endogenous retrovirus (replication competent) .

Endogenous retrovirus (replication competent) RT PCR kit

RTq-V725-150D 150T
EUR 841.2
Description: A Real-Time PCR kit for detection of Endogenous retrovirus (replication competent) .

Endogenous retrovirus (replication competent) RT PCR kit

RTq-V725-50R 50T
EUR 757.68
Description: A Real-Time PCR kit for detection of Endogenous retrovirus (replication competent) .

DH10B Chemically Competent Cells - 12x50µl

1011-12 12/PK
EUR 125
Description: Intact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.Product Includes:ig™ 10B competent cellspUC19 control DNARecovery medium

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