Human T-Cell Cloning by Limiting Dilution

Human T-Cell Cloning by Limiting Dilution

Human T cells symbolize a heterogeneous inhabitants, together with cells with totally different phenotypical and performance properties. Regardless of, within the final years, a number of applied sciences had been developed to analyze phenotypical properties of T cells at single cell degree, in vitro T cell clone ‘s tradition stays the one solution to carry out useful research on T cells at single cell ranges. Right here, we describe the tactic to acquire human T cell clones by limiting dilution within the presence of feeder cells and to take care of them in tradition for additional investigations.

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Fusion (FUS) Antibody

20-abx212424
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Fusion (FUS) Antibody

20-abx172487
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Fusion (FUS) Antibody

20-abx176518
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Recombinant Fusion (FUS)

4-RPC260Hu01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Fusion expressed in: E.coli

Recombinant Fusion (FUS)

4-RPC260Mu01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Fusion expressed in: E.coli

Morpheus Fusion FX

M-MD1-130-FX 96 x 100 ul ul
EUR 67
Description: Morpheus Fusion FX

CD28, Fc fusion

71113 200 µg
EUR 320
Description: Human secreted CD28-Fc fusion protein, also known as TP44, GenBank Accession No. NM_006139, a.a 19-152, expressed in a HEK293 cell expression system. MW = 41.4 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

HVEM, Fc fusion

71142 100 µg
EUR 295
Description: Human herpesvirus entry mediator A, also known as HVEM, TNFRSF14, CD270, and HVEA, GenBank Accession No. NM_003820, a.a. 37-202 fused to human IgG1 Fc, expressed in a HEK293 cell expression system. MW = 44.2 kDa. This protein runs at a higher M.W. by SDSPAGE due to glycosylation.

CD40, Fc fusion

71174 100 µg
EUR 250
Description: Human secreted CD40, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, and Bp50, GenBank Accession No. NM_001250, a.a. 21-193 expressed in a HEK293 cell expression system. MW = 45.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

CD27, Fc fusion

71176 100 µg
EUR 320
Description: Human secreted CD27, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001242, a.a. 21-192 expressed in a HEK293 cell expression system. MW = 45.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

CD47, Fc fusion

71177 100 µg
EUR 325
Description: Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein, antigenic surface determinant protein OA3, antigen identified by monoclonal antibody 1D8, IAP, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system. MW = 40 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

TIGIT, Fc fusion

71186 100 µg
EUR 320
Description: Human T-cell immunoreceptor with Ig and_x000D_ITIM domains (TIGIT), also known as V-set_x000D_and immunoglobulin domain-containing_x000D_protein 9, VSIG9, V-set and transmembrane_x000D_domain-containing protein 3, and VSTM3,_x000D_GenBank Accession No. NM_173799, a.a._x000D_22-141 fused to Fc region of human IgG,_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 39.7 kDa. This protein runs_x000D_at a higher MW by SDS-PAGE due to_x000D_glycosylation.

CD44, Fc fusion

71187 100 µg
EUR 320
Description: Human CD44, also known as epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, or phagocytic glyco-protein I (PGP-1), GenBank Accession No. NM_000610, a.a. 21-220, fused with Fc region of human IgG, expressed in a HEK293 cell expression system. MW = 48.7 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Bovine Ig fraction

31R-1001 10 mg
EUR 136.8
Description: Bovine Ig fraction control or blocking reagent

Chicken Ig fraction

31R-1004 10 mg
EUR 248.4
Description: Chicken Ig fraction control or blocking reagent

Horse Ig fraction

31R-1005 10 mg
EUR 136.8
Description: Horse Ig fraction control or blocking reagent

Goat Ig fraction

31R-1008 10 mg
EUR 136.8
Description: Goat Ig fraction control or blocking reagent

Hamster Ig fraction

31R-1009 10 mg
EUR 165.6
Description: Hamster Ig fraction control or blocking reagent

Rabbit Ig fraction

31R-1018 10 mg
EUR 136.8
Description: Rabbit Ig fraction control or blocking reagent

Rat Ig fraction

31R-1021 10 mg
EUR 138
Description: Rat Ig fraction control or blocking reagent

Sheep Ig fraction

31R-1023 10 mg
EUR 136.8
Description: Sheep Ig fraction control or blocking reagent

Bovine Ig fraction

31R-1025 10 mg
EUR 136.8
Description: Purified Bovine Ig fraction for use as a control or blocking reagent

Chicken Ig fraction

31R-1028 10 mg
EUR 248.4
Description: Purified Chicken Ig fraction for use as a control or blocking reagent

Horse Ig fraction

31R-1029 10 mg
EUR 136.8
Description: Purified Horse Ig fraction for use as a control or blocking reagent

Goat Ig fraction

31R-1032 10 mg
EUR 163.2
Description: Purified Goat Ig fraction for use as a control or blocking reagent

Mouse Ig fraction

31R-1040 10 mg
EUR 180
Description: Purified Mouse Ig fraction for use as a control or blocking reagent

Rabbit Ig fraction

31R-1042 10 mg
EUR 136.8
Description: Purified Rabbit Ig fraction for use as a control or blocking reagent

Rat Ig fraction

31R-1045 10 mg
EUR 138
Description: Purified Rat Ig fraction for use as a control or blocking reagent

Sheep Ig fraction

31R-1047 10 mg
EUR 136.8
Description: Purified Sheep Ig fraction for use as a control or blocking reagent

Immunoglobulin (IG) Antibody

abx024071-200ug 200 ug
EUR 1011.6

Fusion glycoprotein F0 Antibody

48527-100ul 100ul
EUR 399.6

Fusion glycoprotein F0 Antibody

48527-50ul 50ul
EUR 286.8

CD152 Mulg Fusion protein

30R-CD152 25 ug
EUR 805.2
Description: Purified recombinant Human CD152 Mulg Fusion protein

CD137L-muCD8 Fusion protein

30R-AC046 25 ug
EUR 764.4
Description: Purified recombinant Human CD137L-muCD8 Fusion protein

VCAP18/GP125 fusion protein

30-1296 1 mg
EUR 4177.2
Description: Purified recombinant VCAP18/GP125 fusion protein

Human Fusion (FUS) Protein

20-abx168102
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fusion 1 Blocking Peptide

20-abx161514
  • EUR 727.20
  • EUR 1713.60
  • 1 mg
  • 5 mg

Mouse Fusion (FUS) Protein

20-abx066708
  • EUR 844.80
  • EUR 343.20
  • EUR 2598.00
  • EUR 994.80
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fusion (FUS) Antibody (Biotin)

20-abx105013
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Fusion 1 (Fus1) Antibody

20-abx121804
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Fusion (FUS) Antibody (FITC)

20-abx106427
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Fusion (FUS) Antibody (HRP)

20-abx107842
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

RSV Fusion Protein Antibody

abx022172-1mg 1 mg
EUR 1296

RSV Fusion Protein Antibody

abx022173-1mg 1 mg
EUR 1387.2

RSV Fusion Protein Antibody

abx022174-1mg 1 mg
EUR 1296

RSV Fusion Protein Antibody

abx022175-1mg 1 mg
EUR 1296

RSV Fusion Protein Antibody

abx022176-1mg 1 mg
EUR 1296

RSV Fusion Protein Antibody

abx022177-1mg 1 mg
EUR 693.6

RSV Fusion Protein Antibody

abx022179-100ug 100 ug
EUR 978

MPV Fusion Protein Antibody

abx023874-1mg 1 mg
EUR 1178.4

Fusion (FUS) Antibody (FITC)

20-abx271237
  • EUR 594.00
  • EUR 309.60
  • EUR 1746.00
  • EUR 811.20
  • EUR 477.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fusion (FUS) Antibody (Biotin)

20-abx271503
  • EUR 560.40
  • EUR 292.80
  • EUR 1612.80
  • EUR 760.80
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

RSV Fusion glycoprotein [His]

DAG-H10334 10 µg
EUR 868.8

UIM-UBA Fusion Protein

6571-250 each
EUR 326.4

Morpheus Fusion HT-96

M-MD1-130 96 x 1 ml ml
EUR 317
Description: Morpheus Fusion HT-96

ACE2, Fc Fusion (Monkey)

100701-1 50 µg
EUR 490
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.

ACE2, Fc Fusion (Monkey)

100701-2 1 mg
EUR 3200
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.

IL6R, Fc-Fusion (IgG1)

100916-1 100 µg
EUR 320
Description: Human IL-6 Receptor, Genbank # NM_000565, a.a. 20-365 with C-terminal fusion to human IgG1 Fc region, expressed in HEK293 cells. MW = 65 kDa + glycans.

IL6R, Fc-Fusion (IgG1)

100916-2 1 mg
EUR 2720
Description: Human IL-6 Receptor, Genbank # NM_000565, a.a. 20-365 with C-terminal fusion to human IgG1 Fc region, expressed in HEK293 cells. MW = 65 kDa + glycans.

Human LTBR, Fc fusion

71112 100 µg
EUR 310
Description: Human secreted lymphotoxin beta receptor_x000D_(LTBR)-Fc fusion protein, also known as_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_002342, a.a 28-219, expressed in_x000D_a HEK293 cell expression system. MW = 48_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Mouse LTBR, Fc fusion

71122 100 µg
EUR 340
Description: Mouse secreted lymphotoxin beta receptor_x000D_(mLTBR)-Fc fusion protein, also known as,_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_010736, a.a 28-221, expressed in_x000D_a HEK293 cell expression system. MW =_x000D_48.8 kDa (monomer). Endotoxin level_x000D_<0.001 EU/ug.This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Frizzled-1, Fc fusion

71123 50 µg
EUR 325
Description: Secreted frizzled receptor (FZD1)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD1, GenBank Accession No.BC_051271,_x000D_a.a. 73-253, expressed in a HEK293 cell_x000D_expression system. MW = 46.4 kDa_x000D_(monomer). This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Frizzled-10, Fc fusion

71124 50 µg
EUR 325
Description: Secreted frizzled receptor (FZD10)-Fc_x000D_fusion protein and WNT receptor, also_x000D_known as FZD10, GenBank Accession No._x000D_BC_074997, a.a. 21-161, expressed in a_x000D_HEK293 cell expression system. MW = 42.7_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Frizzled-4, Fc fusion

71126 50 µg
EUR 325
Description: Secreted frizzled receptor (FZD4)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD4, GenBank Accession No._x000D_BC_114527, a.a. 37-180, expressed in a_x000D_HEK293 cell expression system. MW = 42.9_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Frizzled-7, Fc Fusion

71139 50 µg
EUR 325
Description: Secreted frizzled receptor (FZD7)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD7, GenBank Accession No._x000D_BC_015915, a.a. 33-185, expressed in a_x000D_HEK293 cell expression system. MW = 43.3_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

BTLA(CD272), Fc fusion

71141 100 µg
EUR 320
Description: Human B- and T-lymphocyte attenuator (BTLA)-Fc fusion protein, also known as CD272, GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.

LAG3 (CD223), Fc fusion

71146 100 µg
EUR 320
Description: Human secreted LAG3, Fc fusion protein, also known as Lymphocyte-Activation Gene 3 and CD223. GenBank Accession No. NM_002286, a.a. 23-450 expressed in a HEK293 cell expression system. MW = 73.1 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

CTLA4 (CD152), Fc fusion

71149-1 100 µg
EUR 150
Description: Human secreted CTLA4 , Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

TIM-3, Fc fusion

71151 100 µg
EUR 320
Description: Human secreted TIM-3, Fc fusion protein, also known as T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, T-cell and immunoglobulin and mucin domain-containing protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, and HAVCR-2. GenBank Accession No. NM_032782.4, a.a. 22-200 expressed in a HEK293 cell expression system. MW = 46.5 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

GITR (CD357), Fc fusion

71172 100 µg
EUR 300
Description: Human secreted GITR, Fc fusion protein, also known as Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, Activation-Inducible TNFR Family Receptor, AITR, and CD357, GenBank Accession No. NM_004195, a.a. 26-161 expressed in a HEK293 cell expression system. MW = 41.2 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

OX40 (CD134), Fc fusion

71175 100 µg
EUR 320
Description: Human secreted OX40, Fc fusion protein,_x000D_also known as Tumor Necrosis Factor_x000D_Receptor Superfamily Member 4,_x000D_TNFRSF4, and CD134, GenBank_x000D_Accession No. NM_003327, a.a. 29-216_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 46.8 kDa (monomer). This_x000D_protein runs at a higher MW by SDS-PAGE_x000D_due to glycosylation.

ICOS (CD278), Fc fusion

71179 100 µg
EUR 320
Description: Human ICOS, Fc fusion protein, also known as inducible T-cell costimulator and CD antigen 278, activation-inducible lymphocyte immunomediatory molecule, AILIM, CD278, and CVID1. GenBank Accession No. NM_012092, a.a. 21-140 expressed in a HEK293 cell expression system. MW = 40.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Human CD226, Fc fusion

71252 100 µg
EUR 320
Description: Human CD226 antigen also_x000D_known as DNAX accessory molecule 1_x000D_(DNAM-1), GenBank Accession No._x000D_NM_006566, a.a. 19-247 fused to Fc region_x000D_of human IgG1, expressed in a HEK293 cell_x000D_expression system. MW = 52.8 kDa_x000D_(monomer). This protein runs at a higher_x000D_MW by SDS-PAGE due to glycosylation.

AFP antibody (Ig fraction)

70R-14345 1 ml
EUR 166.8
Description: Goat polyclonal AFP antibody (Ig fraction)

Guinea Pig Ig fraction

31R-1007 10 mg
EUR 138
Description: Guinea Pig Ig fraction control or blocking reagent

Guinea Pig Ig fraction

31R-1031 10 mg
EUR 165.6
Description: Purified Guinea Pig Ig fraction for use as a control or blocking reagent

Ig lambda C Antibody

47919-100ul 100ul
EUR 302.4

Myosin IG (MYO1G) Antibody

20-abx100607
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Ig lambda C Antibody

20-abx121907
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Myosin IG (MYO1G) Antibody

20-abx129770
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Myosin IG (MYO1G) Antibody

20-abx312931
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Myosin IG (MYO1G) Antibody

20-abx177688
  • EUR 1562.40
  • EUR 744.00
  • 1 mg
  • 200 ug

Ig ELISA KIT|Human

EF007369 96 Tests
EUR 826.8

Recombinant Myosin IG (MYO1G)

4-RPD436Hu01
  • EUR 623.20
  • EUR 290.40
  • EUR 2006.98
  • EUR 748.99
  • EUR 1377.98
  • EUR 492.00
  • EUR 4837.44
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Myosin IG expressed in: E.coli

Recombinant Myosin IG (MYO1G)

4-RPD436Mu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Myosin IG expressed in: E.coli

c-Jun GST Fusion protein

30R-2811 100 ug
EUR 296.4
Description: Purified recombinant Human cJun-GST Fusion protein

Human Fusion (FUS) ELISA Kit

20-abx151603
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Fusion (FUS) ELISA Kit

20-abx154044
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

TCF3 Fusion Partner (TFPT) Antibody

20-abx004953
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

TCF3 Fusion Partner (TFPT) Antibody

abx034315-400ul 400 ul
EUR 627.6

TCF3 Fusion Partner (TFPT) Antibody

abx034315-80l 80 µl
EUR 343.2

POM121 And ZP3 Fusion Protein

20-abx261350
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Fusion Glycoprotein F0 (F) Antibody

20-abx317827
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Fusion (FUS) CLIA Kit

20-abx493573
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Fusion (FUS) CLIA Kit

20-abx493574
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

TCF3 Fusion Partner (TFPT) Antibody

abx238635-100ug 100 ug
EUR 610.8

GSK3 alpha protein (GST Fusion)

80R-2554 100 ug
EUR 296.4
Description: Purified recombinant Human GSK3 alpha-GST Fusion protein

Human Fusion (FUS) ELISA Kit

DLR-FUS-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.

Human Fusion (FUS) ELISA Kit

DLR-FUS-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.

Mouse Fusion (FUS) ELISA Kit

DLR-FUS-Mu-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.

Mouse Fusion (FUS) ELISA Kit

DLR-FUS-Mu-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.

PD-1/PDCD1(Fc fusion)

E21-J97 10ug
EUR 411.6

CFP-LC3 fusion lentiviral particles

LVP399-C 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing a CFP-LC3 fusion target (NM_022818.4), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFP-LC3 fusion lentiviral particles

LVP399-G 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing a GFP-LC3 fusion target (NM_022818.4), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

RFP-LC3 fusion lentiviral particles

LVP399-R 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing a RFP-LC3 fusion target (NM_022818.4), provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CFP-Annexin5 fusion Lentiviral particles

LVP445-C 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing (CFP-human Annexin 5) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFP-Annexin5 fusion Lentiviral particles

LVP445-G 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing (GFP-human Annexin 5) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

RFP-Annexin5 fusion Lentiviral particles

LVP445-R 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing (RFP-human Annexin 5) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CFP-Actin fusion Lentiviral particles

LVP446-C 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing (CFP-human Actin) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFP-Actin fusion Lentiviral particles

LVP446-G 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing (GFP-human Actin) fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Biosynthesis of Lengthy-Chain Polyunsaturated Fatty Acids in Marine Gammarids: Molecular Cloning and Purposeful Characterisation of Three Fatty Acyl Elongases

Lengthy-chain (C20-24) polyunsaturated fatty acids (LC-PUFAs) are important vitamins which might be principally produced in marine ecosystems. Earlier research recommended that gammarids have some capability to endogenously produce LC-PUFAs. This research aimed to analyze the repertoire and features of elongation of very long-chain fatty acid (Elovl) proteins in gammarids. Our outcomes present that gammarids have, a minimum of, three distinct elovl genes with putative roles in LC-PUFA biosynthesis. Phylogenetics allowed us to categorise two elongases as Elovl4 and Elovl6, as they had been bona fide orthologues of vertebrate Elovl4 and Elovl6. Furthermore, a 3rd elongase was named as “Elovl1/7-like” because it grouped carefully to the Elovl1 and Elovl7 present in vertebrates.

Molecular evaluation of the deduced protein sequences indicated that the gammarid Elovl4 and Elovl1/7-like had been certainly polyunsaturated fatty acid (PUFA) elongases, whereas Elovl6 had molecular options usually present in non-PUFA elongases. This was partly confirmed within the useful assays carried out on the marine gammarid Echinogammarus marinus Elovl, which confirmed that each Elovl4 and Elovl1/7-like elongated PUFA substrates starting from C18 to C22E. marinus Elovl6 was solely in a position to elongate C18 PUFA substrates, suggesting that this enzyme doesn’t play main roles within the LC-PUFA biosynthesis of gammarids.

gEL DNA: A Cloning– and Polymerase Chain Response-Free Methodology for CRISPR-Based mostly Multiplexed Genome Enhancing

Even for the genetically accessible yeast Saccharomyces cerevisiae, the CRISPR-Cas DNA modifying expertise has strongly accelerated and facilitated pressure development. A number of strategies have been validated for quick and extremely environment friendly single modifying occasions, and various approaches for multiplex genome modifying have been described within the literature via SpCas9 or FnCas12a endonucleases and their related information RNAs (gRNAs). The gRNAs used to information the Cas endonuclease to the modifying web site are usually expressed from plasmids utilizing native Pol II or Pol III RNA polymerases.

These gRNA expression plasmids require laborious, time-consuming cloning steps, which hampers their implementation for tutorial and utilized functions. On this research, we discover the potential of expressing gRNA from linear DNA fragments utilizing the T7 RNA polymerase (T7RNAP) for single and multiplex genome modifying in Saccharomyces cerevisiae. Utilizing FnCas12a, this work demonstrates that reworking quick, linear DNA fragments encoding gRNAs in yeast strains expressing T7RNAP promotes extremely environment friendly single and duplex DNA modifying. These DNA fragments may be customized ordered, which makes this method extremely appropriate for high-throughput pressure development. This work expands the CRISPR toolbox for large-scale pressure development applications in S. cerevisiae and guarantees to be related for different much less genetically accessible yeast species.

Cloning, Expression, Characterization, and Antioxidant Safety of Glutaredoxin3 From Psychrophilic Bacterium Psychrobacter sp. ANT206

Glutaredoxins (Grxs) are proteins that catalyze the glutathione (GSH)-dependent discount of protein disulfides. On this research, a Grx-related gene (264 bp), encoding a Ps-Grx3, was cloned from Psychrobacter sp. ANT206. Sequence evaluation indicated the presence of the energetic web site motif CPYC on this protein. Homology modeling confirmed that Ps-Grx3 had fewer hydrogen bonds and salt bridges, in addition to a decrease Arg/(Arg + Lys) ratio than its mesophilic homologs, indicative of an improved catalytic capability at low temperatures. Web site-directed mutagenesis demonstrated that the Cys13, Professional14, and Cys16 websites had been important for the catalytic exercise of Ps-Grx3, whereas round dichroism (CD) spectroscopy confirmed that time mutations in these amino acid residues led to the loss or discount of enzyme exercise.

Moreover, evaluation of the biochemical properties of Ps-Grx3 confirmed that the optimum temperature of this enzyme was 25 °C. Importantly, Ps-Grx3 was extra delicate to tBHP and CHP than to H2O2, and retained roughly 40% exercise even when the H2O2 focus was elevated to 1 mm Concerning substrate specificity, Ps-Grx3 had the next affinity for HED, L-cystine, and DHA than for S-sulfocysteine and BSA. We additionally investigated the DNA-protective capability of Ps-Grx3 utilizing the pUC19 plasmid, and located that Ps-Grx3 may shield supercoiled DNA from oxidation-induced harm at 15°C for 1.5 h. This research gives new insights into the construction and catalytic exercise of a cold-adapted Grx3.

Molecular cloning and useful research on magang goose toll-like receptor 5

Illness outbreaks closely influence the financial viability of animal industries. Little is thought in regards to the mechanisms of immune system-related illnesses in geese. Toll-like receptors (TLRs) play a significant position within the anti-inflammatory immunity course of in most animal species, however they haven’t been studied within the Magang goose. To elucidate the position of TLRs, reverse transcription polymerase chain response (RT-PCR) and PCR amplification of cDNA ends (Good RACE) had been used to clone the Magang goose TLR5 gene (mgTLR5). The total-length cDNA of mgTLR5 was 2967 bp in size, together with a 5′-terminal untranslated area (UTR) of 215 bp, a 3′-terminal UTR of 384 bp, and an open studying body of 2583 bp that encodes a protein of 860 amino acids. Structurally, mgTLR5 has a toll/interleukin-receptor (TIR) area, a transmembrane area, and 7 leucine-rich repeats (LRRs) domains.

Homology alignment of TLR5 and its TIR domains with different species revealed that mgTLR5 shared 98 % and 81.3 % of sequence similarity with white goose TLR5 and hen TLR5, respectively. Quantitative RT-PCR confirmed that the mgTLR5 gene of the goose is broadly expressed in all examined tissues, with the best expression within the kidney and spleen. The rise in NF-κB promoter exercise stimulated by flagellin was depending on mgTLR5 expression in 293 T cells. Salmonella pullorum and flagellin considerably upregulated the expression of TLR5, IL-8, and IL-1 mRNA in peripheral blood mononucleotide cells of Magang goose cultured in vitro. Stimulation by S. pullorum for 24 h upregulated mgTLR5 expression within the cecum and kidney. We conclude that Magang goose TLR5 is a useful TLR5 homologue of the protein in different species and performs an essential position in bacterial recognition.

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